SERUM or PLASMA, which could be better choice to isolate viral RNA ?

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Elamurugan Appavoo

thank you sir.. still i tried twice with plasma samples RNA concentration 9 - 10 ng/micro litre

was very low and purity was worse.. i am using QIAGEN viral RNA isolation kit....

Thank you sir..

Santhosh Bailur

serum will be good sample for RNA virus isolation but sensitivity will be depend on time of blood collection. more sensitive during early course of the infection up to one week.

Liubov Yurjevna Vergun

Certanly plasma is better because in process coagulation (receiving of serum) some part of virus will be in a lost with fibrin.

Michel Segondy

EDTA plasma is better. Serum can be used for viruses giving a high viremia level such as parvovirus B19, HBV,...