How can I make a stable HEK293 cell line using pcDNA3.1?

I wish to make stable HEK293 cells with my gene of interest using pcDNA3.1 How can I proceed?

11 December 2014 8,562 17 View

How do I get good yield of eukaryotically expressed proteins?

11 December 2014 9,665 2 View

How do I confirm eukaryotically expressed proteins?

I have cloned my protein into mammalian expression vector pVAC1 and subsequently transfected 6 ug of DNA into 293 cells using lipofectamine...protein will be secreted out of the cell, since vector...

09 October 2014 6,749 7 View

How to add a secretory signal to a protein?

i have a protein that is not naturally secreted out of the cell..so i decided to add a secretory signal (eg. IL-2 signal sequence) at 5' end of the gene... kozak-secretory signal-my gene of...

09 October 2014 1,200 3 View

How does one isolate cytoplasmic and nuclear proteins from cell line (without kits)?

08 September 2014 5,920 7 View

Can anyone suggest how to dissolve TCA precipitated proteins?

I have precipitated cell culture supernatants with TCA (10%) followed by overnight incubation and acetone washing...then I have added Lammeli sample buffer, but I am unable dissolve the protein...

08 September 2014 2,010 17 View

Cloning C-terminal GFP to protein - will this interfere with the expression of GFP?

I have cloned GFP at C-terminal of my protein to confirm its expression by GFP fusion After end of my protein 2 aminoacids are present before start of GFP I wanted to know whether this 2 a.a will...

07 August 2014 3,779 9 View

Transfection of BHK 21 using Lipofectamine LTX plus reagent?

I am doing transfection of BHK 21 cells using Lipofectamine LTX plus. My vector is pVAX1, but I am not getting the expressed protein I included kozak sequence before my gene. The protocol which i...

02 March 2014 4,616 4 View

Can anyone suggest a signal peptide for secretion of a recombinant protein of bovine origin?

I wish to express a recombinant protein that is not naturally secreted. How to signal a sequence so that it can be secreted out?

11 December 2013 4,884 1 View

Isolation of bovine bone-marrow monocytes?

How to isolate monocytes from bone marrow? I need to further culture them for in vitro studies.

11 December 2013 3,405 5 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Theoretical computer virology ? is still alive ?

HI Everyone! I am asking to know by you, expert in cybersecurity and mathematics, if Computer virology (the one of Cohen and Andler) is still an active field of research. That research made in...

08 February 2021 7,613 6 View

Serum causes Hemagglutination in HI test. Virology Expert Please help how to resolve this issue while doing HI test for NDV and Avian Influenza?

I am doing HI Test for NDV and Avian Influenza Viruses but before going to start I first tested the serum whether any reactivity exists or not with 1% washed Chicken RBC. but I found that some...

11 January 2021 1,043 7 View

Can you trust vaccines that were made in a year, will help fight Sars-Cov2?

That vaccines have been produced in such a short time, makes me doubt if they will help fight this pandemic and if they will not do us more harm.

06 January 2021 3,469 19 View

Any papers discussing the effect of Zika virus on neuron development and its effects on neuronal functions?

I am looking into this research, any interesting papers relating to this would be amazing. Papers on pregnant women and infants would be great. Thank you :)

30 December 2020 1,962 3 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

How can chemistry help fighting COVID-19?

Chemistry is frequently termed the "central science". As such, chemistry is expected to play a major role in fighting the coronavirus. For example, hand sanitizers and drugs are all based on...

30 November 2020 4,983 17 View

What is the scope of doing PhD in Virology in the UK?

With the great interest in Virology in the world due to the COVID-19 pandemic and its related vaccine development is doing a Phd in Virology from a good institute in UK the right thing to do?

26 November 2020 3,681 3 View

Who can collaborate with us?

Salam (Hello)! We have prepared some new compounds with important potential to inhibit viruses. We are interested in inhibition of Hepatetis-B/C, Nipah, Nil and coronavirus. Who can collaborate...

22 November 2020 3,850 14 View

Biotech Startup Research?

26 October 2020 9,698 5 View