17 Questions 16 Answers 0 Followers
Questions related from Elamurugan Appavoo
I wish to make stable HEK293 cells with my gene of interest using pcDNA3.1 How can I proceed?
12 December 2014 8,771 17 View
i wish to express a protein eukaryotically in good quantity? which system i can go for? adeno viral vectors are good choice whether these vector have fusion tags to purify? (except yeast...
12 December 2014 9,697 2 View
I have cloned my protein into mammalian expression vector pVAC1 and subsequently transfected 6 ug of DNA into 293 cells using lipofectamine...protein will be secreted out of the cell, since vector...
10 October 2014 6,776 7 View
i have a protein that is not naturally secreted out of the cell..so i decided to add a secretory signal (eg. IL-2 signal sequence) at 5' end of the gene... kozak-secretory signal-my gene of...
10 October 2014 1,229 3 View
I have precipitated cell culture supernatants with TCA (10%) followed by overnight incubation and acetone washing...then I have added Lammeli sample buffer, but I am unable dissolve the protein...
09 September 2014 2,044 17 View
Can anyone suggest a protocol for extraction of nuclear and cytoplasmic proteins from cell line without kits and detergent solution (NP-40)... Can I use Nuclear Extract (NE) buffer (HEPES [20 mM]...
09 September 2014 5,955 7 View
I have cloned GFP at C-terminal of my protein to confirm its expression by GFP fusion After end of my protein 2 aminoacids are present before start of GFP I wanted to know whether this 2 a.a will...
08 August 2014 3,813 9 View
I am doing transfection of BHK 21 cells using Lipofectamine LTX plus. My vector is pVAX1, but I am not getting the expressed protein I included kozak sequence before my gene. The protocol which i...
03 March 2014 4,652 4 View
I wish to do in vitro studies to evaluate my protein efficiency to stimulate immune system in relation to bovine animals.
12 December 2013 2,420 4 View
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12 December 2013 2,259 5 View
I have a sequence of my protein but I don't know whether it has a signal sequence for its extracellular secretion. If it does not, what signal sequence do I need to add for the secretion?
12 December 2013 7,512 6 View
I wish to express a recombinant protein that is not naturally secreted. How to signal a sequence so that it can be secreted out?
12 December 2013 4,917 1 View
How to isolate monocytes from bone marrow? I need to further culture them for in vitro studies.
12 December 2013 3,437 5 View
MAb available for mouse protein and I would like to know whether it will work in pig other any other animal?
11 November 2013 9,400 2 View
I would like to know what are CD markers for bovine DC.
11 November 2013 294 0 View
They have different promoters and my protein needs to be secreted out of the cells. Which one would be a better choice?
11 November 2013 7,598 0 View
I am trying to isolate viral RNA from suspected AVIAN LEUKOSIS VIRUS positive birds, whether i can go for serum or plama
05 May 2012 9,098 5 View
