For sequential infection of two different lentiviral vectors into mammalian cells, is it possible that introduction of the 2nd viral vector can cause the lost (to some extent) of the previously introduced gene(s)?
John Schloendorn
Sure. Expression should decrease "to some extent" when you just keep the cells in culture. It can vary from mild to dramatic. Anything that increases stress on the cells should accelerate the losses. This very much includes a second infection.
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