Seeking Assistance with NBD-DPPE Lipid Purification, NMR Sample Preparation, and Acquiring NMR Data?

13 July 2023 1 5K Report

Dear ResearchGate Community,

I hope this message finds you all well. My name is Michael G. , and I am a Ph.D. student. We are currently synthesizing the lipid NBD-DPPE and are facing some challenges concerning its purification, NMR sample preparation, and in need of NMR data.

Purification: We're looking for effective methods to purify NBD-DPPE. Any advice regarding techniques, tips, or even literature recommendations would be greatly appreciated. In particular, we'd like to know details regarding column chromatography (mobile-phase, stationary phase, etc)

NMR Sample Preparation: We would also appreciate guidance on preparing the NBD-DPPE lipid sample for NMR analysis, including the most suitable solvent system and ideal concentration. We understand that lipids can sometimes present specific challenges in NMR analysis due to their hydrophobic nature and tendency to aggregate, and so any advice on this matter would be highly valuable.

NMR Data: Lastly, if anyone has NMR data of NBD-DPPE lipid and would be willing to share, this would immensely help us in validating our results and ensuring the accuracy of our product.

Thank you very much for your time and consideration. I look forward to any advice or suggestions the ResearchGate community may have to offer.

Best regards,

Michael G. Ph.D. Student.

Luc Alders

I can't help you with your purification or reference spectra, but we do occasionally work with comparable lipids and they tend to dissolve relatively well in methanol/chloroform mixtures.

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