How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Which Tm should I use in my PCR ?

I have ordered primers from Thermo fisher scientific and in the analysis sheet it has been mentioned two tm The first one is tm 1M na+ 70 and the second is Tm 50mM na+ 48 and each has different...

29 August 2020 6,890 12 View

How i can Improve the accuracy of an artificial intelligence model for test data in MATLAB ?

Hello everyone, I have a question about artificial intelligence in MATLAB. Can you help me, to improve the accuracy of an artificial intelligence model for test data in MATLAB software? (while...

22 July 2020 2,692 12 View

Does anyone know how can I get Mito Thermo Yellow (MTY) dye?

This is a thermosensitive dye that was recently used by chretien et al to measure changes in mitochondrial temperatures.

01 March 2020 4,863 4 View

If a staph bacteria shows resistance to vancomycin disk on Mueller Hinton plates, does it mean its Methecillin resistance?

Also can staph aureus bacteria appear pink on Manitol salt agar plates? Or it definitely appears yellow? Has anyone isolated staph aureus with alpha hemolytic characteristics? Thanks.

06 February 2019 409 4 View

What kind of emulsifier you propose for emulsion-based drinks with acidic pH having long shelf life?

Beside lecithin, some kind of sucrose ester FA, whey protein, do you know some kind of emulsifiers that are able to produce a long-term stable O/W emulsion ?

18 December 2018 2,759 4 View

How to use yalom existential psychotherapy or group therapy in school setting?

Psychotherapy can help most patients confront the issue of responsibility for their own life, problems, and improvement (Yalom, 1974). Group therapy can be somewhat beneficial for clients who are...

12 December 2018 6,850 3 View

What are the safety requirements when using nano-carbon tubes for reinforcing Polymeric matrix?

Hi for All I need your recommendations from safety requirements when using nano-carbon tubes for reinforcing Polymeric matrix. Thanks in advance

06 July 2018 326 2 View

Do you know the latest list of the journals that discontinued from Scopus?

Hi dear colleagues Do you know the latest list of the journals that discontinued from Scopus? You can see it in this file which downloaded from Scopus website (last updated 26/3/2018). Regards

14 April 2018 4,977 17 View

From the copyright viewpoint, is it legally permitted for the researcher to add the full text of the published paper on the research gate platform?

Hi dear colleagues. From the copyright viewpoint for journals, is it legally permitted for the researcher to add the full text of the published paper on the research gate platform? This question...

05 April 2018 9,253 74 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

How can I label an antibody to determine how far it is travelling from the microdialysis probe?

I am trying to set up a new experiment where I am simultaneously utilizing reverse dialysis (antibody) through a microdialysis probe and a Miniscope for one-photon calcium imaging. Both pieces of...

09 February 2021 3,981 1 View

QPCR troubleshooting - what could cause an increasing ROX signal?

Hi everyone, I'm looking for some help troubleshooting a recent qPCR issue I've encountered. I am using fluorescent Taqman probes and a qPCR master mix that includes ROX as a passive reference....

08 February 2021 419 3 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

Is it strictly recommended to run samples in duplicate during SNP genotyping analysis?

02 February 2021 8,877 3 View

How can I get access of UNAfold tool?

I am searching for tools to construct molecular beacons which shall be complementary to a given sequence. I don't want to design any primers. I found that UNAfold is one such tool which I could...

02 February 2021 9,065 5 View

Is there a comprehensive database for Virus Real-Time Primers and Probes ?

Dear All I am looking for a database for tested Real Time PCR primers and probe sequences for the following viruses for diagnostic purposes. Does the USAID PREDICT project or the Global Virome...

01 February 2021 8,920 1 View