Kamelia Mirdamadi , 5-6V/cm refers to the voltage gradient across the electrophoresis unit. That is, you measure the distance between the electrodes (in cm) and multiply that by the provided value. In your case, if the electrophoresis unit has, say, 20 cm between electrodes, then it should be run at 100-120 V.

Also, what do you mean by weird results? What type of sample are you analyzing (total RNA? synthetic ssRNA?) and from where?

Hope it helps!

Are you sure it is RNA you wanted to visualize on gel?

Hello,

I agree with

Here in the lab, we use by 110 to 140V with no problems, you should always use some marker, like 1Kb or 1KbPlus, and I have some doubts about it being RNA too.

I recommend you to visualize the gel with 5', 10' and 15', cause the sample changes itself in the way to the end of the pool, the bands change with time.