Kamelia Mirdamadi

3 Questions 1 Answers 0 Followers

Questions related from Kamelia Mirdamadi

Any suggestions on the effect of 1mM sodium citrate solution on reverse transcritpion?

I started using "RNA storage solution (1mM sodium citrate) from THermoFisher to dissolve RNA pellets and store in -20 or -80. However, there is no amplification in the Q-PCR. it is frustrating and...

06 November 2019 9,126 3 View

Concentration calculation in multi-analyte ELISA?

Hello, I did multi-analyte ELISA to detect the concentrations of several cytokines, but how should I calculate the concentration as each well represents a different sample and a different...

12 July 2019 9,799 2 View

RNA Quality and formaldahyde Agarose gel?

I am trying to run RNA on 1% agarose/formaldehyde gel using 5x MOP buffer and SybrSafe staining instead of ethidium bromide. what is the proper voltage and time? it says 5-6V/cm, but how can I...

01 January 1970 8,247 3 View