DNA/RNA G4 modelling and visualization tool ?

Hi, I would like to ask if anyone knows any tool for visualizing/modelling guanine structures (any RNA structures with Hoogsteen base pairing) based on sequences? I mean, if there is any...

02 November 2020 1,136 2 View

Does anyone have any experience with rRNA depletion prior to transcriptome NGS?

16 October 2016 5,033 3 View

Is there any chance to melt Ni3Al dice (4,5 mm) on oxide ceramics in resistance furnace under Ar atmosphere ?

We used a lot of types of oxide ceramics based on Al2O3 and even used Spinel (MgAl2O4) ceramics - refractory concrete, which is even more stable, but Ni3Al always oxidized and not melt. We are...

09 February 2016 6,274 3 View

Is anyone interested in collaboration in the area inquiry-based learning?

I am interested to scientifically collaborate (projects, comparative research, training) on the topic of "inquiry-based learning" - IBSE. Anyone interested?

15 June 2015 5,340 3 View

Are there specific photographic techniques of surveys on for public perception of forest?

I prepare questionare surveys about public perception of forest within project http://rafa.frrms.mendelu.cz/en?lang=en. I have ideas about objectives of questionare, but Im looking for rules...

26 March 2015 2,524 3 View

What is the least drastic method to kill decapod crustaceans for subsequent preservation?

A variety of methods are used to kill decapod crustaceans for subsequent preservation. Generally the most acceptable method of killing crustaceans is to chill the animals to the insensitivity and...

24 October 2014 2,021 20 View

Are we testing the impact of medical drugs on the health of gut microbiota?

The current research is showing a great impact of gut microbiota on human and animal health. The question is: "Are we developing methodologies and approaches that enables us to test impact of...

01 January 1970 2,425 5 View

Is Self-organization and Emergence in Biology and Medicine Interesting subject for You?

Dear Colleaugues, There was a proposal of a researcher who asked me to write down a paper which will more or less extend the poster (link bellow) published at ICCB 2016 in Prague. This all...

01 January 1970 7,948 10 View

How to plan sample size for a crash prediction model?

Can somebody help estimating how many analysis units (number of railway level crossings - RLCs) will I need to build a crash prediction model (safety performance function)? From some past analyses...

01 January 1970 3,615 3 View

How is the use of hand-sanitizers influencing the overall health of whole populations?

We all know that poor hygiene within dense populations is a breeding ground for diseases. On the other hand, we know that some kind of medium level of exposure to infectious agents on a daily...

01 January 1970 1,922 10 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View