What are your tips to write the discussion section of your scientific work in an efficient way?

Writing the discussion section is always the scariest part of a paper or a thesis. I came up with some ideas to make it less difficult, but would like to hear other tips! Here are my two bits:...

04 November 2020 6,808 3 View

How can I determine the total antibodies in the serum of bovines with ELISA?

I want to determine if there is a difference in the amount of total antibodies in the serum of different bovines, I was thinking of doing it with Elisa sandwich. Has anyone tried it? What initial...

13 August 2020 2,412 4 View

What measures have your labs/ institutions put in place to mitigate the impact of COVID-19 on early career-researchers?

Early career-researchers are disproportionately affected by the COVID-19 pandemic in academics, as they often struggle with precarious working situations and they are seeing many doors shutting...

27 May 2020 1,440 1 View

Avigan ( Favipiravir), Hydroxychloroquine plus Azithromycin. A possible Cocktail against SARS-COV2 ?

All the three medicaments have promising results reported and supposed complementary mechanism of action?

10 April 2020 6,909 7 View

In Limma package, why should I linearize my data in some analyses?

tl;dr: Why data linearization is applied in the identification o differentially expressed genes/proteins? I am new to data analysis of big data like proteomics. As far as I know, a simple t-test...

16 November 2019 8,441 4 View

What are the do's and don'ts of good research dissemination plans?

Funding agencies increasingly ask for research dissemination plans, in particular in public health, and some require that a considerable amount of time in your project is solely dedicated to this...

17 October 2019 7,557 3 View

Does anyone know of UV transparent 48 well microplates and adequate to cell culture?

I need to perform experiments with UV irradiation at 270 nm that require a 48 well plate layout. Does anyone know of UV transparent microplates with less than 96 wells? Besides, are there any UV...

05 June 2019 6,627 2 View

Is there anyone who works with ImageJ/Skeletonize3D for quantifying microglia morphology from photomicrographs?

I am having some troubles at the end of the analysis and I guess is due to my version of ImageJ 1.45S. Which version of the plugin Skeletonize3D and AnalyzeSkeleton should I use? I have tried a...

05 April 2019 632 2 View

Could we organize collective human intelligence to achieve and surpass the breakthroughs obtained or crystallized by individual human geniuses?

The biophysicist Jacques Monod hypothesized that man will never understand itself due to that a logical system could not be higher than itself. With the internet becomes common use the somewhat...

30 January 2019 8,014 10 View

Are strain rate effects automatically considered in ABAQUS?

Hi, I'm using ABAQUS to perform a dynamic explicit analysis and I would like to know if strain rate effects in the material behavior are automatically considered by the software. My model involves...

23 August 2018 7,949 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View