Karrie Goglin-Almeida

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Questions related from Karrie Goglin-Almeida

RNA input into SARS-CoV-2 qPCR?

I'm trying to determine how much RNA, extracted from a nasal swab, should be used for qPCR. Many of the papers only give a volume, usually 5uL. Does 5uL correspond to 10pg, for example? Since the...

25 March 2021 946 1 View

Why is the qPCR concentration sometimes much higher than Qubit concentration for some Illumina libraires?

We use the NEBNext Library Quant Kit for Illumina to determine our Illumina library concentrations. Before taking the library to qPCR, we usually run the library on Bioanalyzer to get an idea of...

28 January 2021 7,119 4 View