RNA extraction from paraffin embedded tissue ?

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How we can dissolve theophylline derivatives for the MTT test?

I have a question about your letter, Fluorescent phosphoinositide 3-kinase inhibitors suitable for monitoring of intracellular distribution. Can you tell me how we can dissolve theophylline for...

26 January 2021 9,094 1 View

Can anybody suggest me a cheap material for surface modification of hydrophilic Titania nanoparticles?

I'm looking for a cheap material to modify Titania nanoparticles in order to use them in polymer and increase its contact angle.

13 December 2020 504 5 View

Importance of the tunnel effect on the solar cells performance?

Does the tunnel effect play a major role in the performance of solar cells? Especially when the device is heavily doped so the depletion layer can be thin enough for tunneling. Are there any...

10 December 2020 5,518 10 View

In XPS analysis of GO films. How I can use the sample work function method to know the information of XPS binding energy scale is referenced ?

Note that aligning the spectra to the C 1s peak of adventitious carbon is a highly risky procedure and was recently shown to lead to unphysical results. Therefore, I strongly suggest to avoid it....

18 November 2020 5,483 4 View

Dear Doctors/Professors, i would like to ask if a novel drug would be tested in animal model in mice which range of doses do i use first ?

03 August 2020 3,811 3 View

How can I find a questionnaire for Corporate identity?

My thesis is about the relation between corporate social responsibility and corporate identity. And I can not find any questionnaire about it. Can you help me?

24 July 2020 3,507 3 View

When I injected it into the LCMS all the chromatogram peaks are small lthough I have increased the concentration?

I have a metabolic extract from a plant, when I injected it into the LCMS all the chromatogram peaks are small, although I have increased the concentration several times but there is no change in...

12 March 2020 3,047 2 View

How to deal with a skewed Likert scale data with Ceiling/Flooring effects in a before-after design?

I have data of 30 subjects from a before-after design study. We used likert scale to investigate effects of changes in mood on some dependent variables. We hypothesized that the intervention would...

22 February 2020 6,496 5 View

What are the quantities per microliter of the different components needed for a reaction (1isolate of S. aureus ) to run a multiplex PCR ?

Dear colleagues, I need help. I want to run a multiplex PCR to look for enterotoxin-encoding genes in coagulase-positive StaphyloccIoccu . I have all the reagents needed to prepare the MIx PCR...

13 February 2020 7,036 2 View

How can I do the Magnetic calculations using CASTEP code as ferromagnetic system , anti-ferromagnetic system ?

with a CASTEP

01 February 2020 1,612 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Nour Eissa

In my lab, we usually use FFPE kits from thermofisher with good RNA quality and qunatity. No tricks, just follow the kits’ instructions and you will be good to go

Here is the link:

https://www.thermofisher.com/ca/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-sample-extraction.html?open=ffpe#NaN

Leila Abdelhamid

Thanks Dr. Nour Nour Eissa @ :)