RIN value is greater than 7 but rRNA ratio < 1?

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Interested in joining us as an editor?

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How to prepare chitosan solution 8% in TFA:DCM??

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Sylvester Ampem Obeng-Darko

I believe your RNA extract is intact. In some RNA extract the 23S and 28S rRNAs contain AU-rich sequence called hidden breaks that can lead to processing RNA into smaller RNAs. So when you strike the ratio of 28S : 18S or 23S : 18S the resulting value could be

Dania Saeed

Thank you for weighing in Sylvester. Hope so as well :)