Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How or where can I get the crystallographic information file (CIF) for PCN-222 MOF?

I need the simulated XRD pattern of PCN-222 to compare with experimental data. Thanks.

19 February 2021 4,932 3 View

Websites for virtual lab in semiconductors?

Hello everyone, Because of Covid, now all classes are online. Is there good website that also are easy to use to teach student virtual experiments about PN junctions and semiconductors? Thank...

24 December 2020 6,637 2 View

From the EDAX analyses if the material which is coated is not observed in maximum percentage then how can interpret the results?

If we doped metal oxide to semiconducting material in different ratios then the property of the resultant material will be increased.But in some lower ratios when we go through the elemental...

16 December 2020 8,190 2 View

Is there any software for calculating hit bias (HB), missed precipitation (MP), and false precipitation (FP)?

I have a time series of two rainfall products. For error decomposition analysis, I have to calculate HB, MB and FB. Is there any software for calculating these variables.

27 November 2020 980 2 View

Why Schiff bases change colour upon evaporation?

I got bright yellow coloured Schiff base after 6 hour reaction time. So i kept it for slow evaporation. Solvent was methanol. But after complete evaporation my product colour changes from yellow...

17 November 2020 3,824 2 View

How to make the children cool, who feel stressful during this pandemic-lock down without having physical outlet?

The discussion may feed the parents a way to manage and guide the children in a right direction.

12 November 2020 1,302 9 View

How to design qPCR primer for mature microRNA?

09 November 2020 9,163 3 View

Which force field should be used to simulate protein-protein complex in gromacs?

Hi, I have done a protein-protein docking. Now I want to perform MD in gromacs. I am confused with forcefields. When I tried to findout, everywhere it is written for protein-ligand but I couldn't...

29 September 2020 1,410 3 View

Charcoal mediated steroid depletion assay?

Suppose, one is doing enzyme assay of an enzyme of a steroidogenic pathway. Is it necessary to deplete the steroids from the tissue/ cell homogenate before administering substrates to check enzyme...

21 September 2020 7,439 2 View

Is there anybody to share a RevMan tutorial file with me?

I am busy writing a meta-analysis manuscript. I had passed a RevMan course previously, however, I need to be refreshed again.

21 September 2020 3,535 5 View

How to calculate the pooled complication rate for a meta analysis ??

I am helping in writing a systemic review and Meta analysis. I need to calculate the complication rate. what i have is ?? Study, number of procedures done, number of complication observed and...

06 July 2020 8,690 1 View

SD of Change for Meta-Analysis?

I am looking to do a meta analysis on intervention RCTs but all of the papers have provided baseline and post intervention Mean (SD) for the groups. I have looked at the Cochrane page as I am...

27 March 2020 4,781 3 View

Is it possible to make a forest plot in RevMan using an analysis I've done separately?

Hi all, I am currently conducting a meta analysis. I've done the actual analysis on CMA (on the free trial, the program better suited my study), however, I would now like to use this data to...

22 March 2020 9,908 1 View

Hi Guys, I am doing a meta-analysis of fertilizer response studies (eg. Effect of different nitrogen rates on yield). How to enter data in RevMan?

How to enter the different fertilizer rates (including a control) in RevMan?

02 March 2020 5,309 2 View

How can i combine multiple standard deviations in a meta-analysis?

I need your help. Maybe it's a simple question, but i'm new to this stuff. I'm doing a meta-analysis (with RevMan) and in some studies i have standards deviations from pre and post intervention ....

11 December 2019 8,493 4 View

How to meta-analyse data from one arm pretest-posttest quasi-experiments?

Hello, I have 3 one-arm pretest-posttest quasi-experiments. Is it possible to do meta-analysis to their findings? If so, how can I analyse them using Revman? For me, I considered pretest data...

03 July 2019 5,354 4 View

Hi, i have created table of characteristics of included studies in RevMan, now i want to get this table in my word file,so guide me how i can do this?

I'm writing systemic review so i need some guidance related to use of RevMan, how i can get tabulated data in word file from revman??

20 May 2019 4,731 2 View

How can I change the color of a forest plot in RevMan?

Some journals charges a fee when you have color figures. I want to change forest plot into grayscale version. How can I do it?

16 May 2019 9,691 2 View

Can I perform meta-analysis when the exposure veriable is continous?

I am hoping to undertake my first meta-analysis, where the exposure variable it mean number of decayed, missing or filled teeth)and the outcome is BMI category (underweight, normal weight,...

09 May 2019 8,757 4 View