Has anyone had success with retrograde tracing of peripheral axons using FluoroGold but without perfusing the animal with paraformaldeheyde at the end of the timepoint?
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
05 August 2024 2,333 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.
05 August 2024 1,842 3 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View
Comprehensive Overview of Myocardial Infarction: Etiology, E... What imaging modalities are useful in assessing myocardial perfusion and viability?
15 June 2024 3,019 2 View
I am attempting to model a Concentrated Solar Power (CSP) model with heliostats and a receiver tower within SolTrace. When altering the heliostat coordinates, the ray tracing indicates that...
12 May 2024 4,012 1 View
I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...
02 May 2024 4,928 0 View
I have tried the attached protocol to isolate primary hepatocyte from mice, using Collagenase instead of Liberase. Everything is the same as mentioned in the protocol. Except the following : Used...
30 April 2024 8,841 1 View
Hi! I am looking for an open-source tool to analyse perfusion CT scans from the hospital. I would like to assess CBF, ATT and permeability maps. Do you have any suggestion? until now, I have been...
14 April 2024 9,974 0 View
Good morning, I need to isolate dendrites from axons in my microchamber, I have already tried a protocol of Netrin and it failed. I would like to put Sema3a in my dendritic compartment in order...
26 March 2024 2,632 0 View
I have tried isolating ventricular cardiomyocyte by perfusing type II collagenase through adult mice hearts. When looking at the extracted cells under 20X magnification, I don't see the typical...
21 March 2024 3,725 1 View
I want to examine the correlation between signal intensity of Hoffa's fat pad and KL grades of knee osteoarthritis. But i am not able to get how to measure the signal intensity. Elliptical/round...
11 March 2024 9,693 1 View
https://wikis.utexas.edu/display/khlab/Transcardiac+Perfusion In this article, they mention that the rat should be kept for 1hr before dissecting. I, however, do not do that in my lab. Is this a...
01 March 2024 9,148 2 View
Excuse me. I'm currently doing experiments on ray tracing in indoor environments. But I couldn't find a 2D data set for complex indoor environments. You know, the format of the data set that I'm...
29 February 2024 4,767 0 View