Do we keep the rat for 1hr after the fixation by perfusion is done before dissecting out its brain?

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Samir Ranjan Panda

Hi Sakshi Sharma

No, it is not recommended in general. We isolate the brain immediately following perfusion and store in PFA.

Thanks,

Louis-Charles Béland

Hello Sakshi,

If you transcardiacally perfuse your animal with PFA, the brain will be fixed. You don't have to wait 1hr for the brain dissection but if you prefer to perfuse all your animals and then dissect after, you can certainly wait 1hr between the perfusion and the dissection