We hypothesized that resveratrol, a natural phytoalexin found in grapes, can
prevent oxidative stress, obesity and its related disturbances in obese rats
programmed by early weaning. Does anyone have any experience with the methods used to assess oxidative stress as TBARS, superoxide dismutase (SOD) and liver glutathione peroxidase activities ?
I want to know mainly, what you use for formation of thiobarbituric acid-reactive substances (TBARS).
Thank you very much.
Ok I'll read the paper and thank you for your attention and help.
Obviously, besides its effect on Sirt1; resverarol has a number of antioxidants effects which might have an impact on tissue inflammation and oxidative stress observed in a number of tissues in obesity. There are a number of techniques to quantify oxidative modifications of macromolecules, refereed as oxidative stress. There is a compendium on techniques to assess oxidative stress in a special issue in Free Radicals Biology and Medicine published in 2009 that might be of your interest.
Maybe you are interested in a recent meta-analysis reporting results on the proposed life-extanding effects of resveratrol (http://rsbl.royalsocietypublishing.org/content/early/2012/06/13/rsbl.2012.0316.short). Also, the cited references might be a starting point for the methods of measuring different oxidative stress markers.
Magna, we have very problems with TBARS. Can you tried 4-HNE detection? I think this is a better marker for lipid peroxidation.
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