I am using a protocol to screen plant extracts against yeast C. neoformans. The protocol states that extracts should be resuspended in acetone to 10 mg/mL because bacteria and yeast can mostly survive acetone concentrations up to 25% wheres the same cannot be said for other solvents.

I have problems dissolving my methanol extracts in acetone eppendorf tubes. I try my best to break up the hard parts with a pipette tip and vortexing but still dont manage to always break the larger parts up.  And since the hard parts make up the largest mass of the 10 mg/mL when resuspended in aceetone - surely my MIC values are then worse (higher) than they should be if all the extract dissolved in acetone.

I would just like to know if you know of a solution to this problem.