Chris la Grange

6 Questions 11 Answers 0 Followers

Questions related from Chris la Grange

TargetLynx LCMS: Is it possible to quantify all compounds from 140 samples without manually inputting each compound into a method?

I have 140 samples from (50 plant species) analysed by LC-MS. Is there a way in which I could use TargetLynx (MassLynx 4.1) to automatically quantify all the compounds in all 140 samples that...

17 May 2018 8,001 2 View

MassLynx - Markerlynx statistics question: averaging of technical repeats?

I performed triplicate extractions (technical repeats) from the same batch of plant material. This was done for 40 plant species. Each of the triplicate extracts were analysed via LCMS. I...

15 March 2018 7,887 3 View

I need advice on enriching a methanolic plant extract through selective extraction to improve MIC's against Candida albicans?

I have approximately 150 grams of dried plant material (Pelargonium). I have already determined the methanol extract to have significant MIC values against C. albicans. I would like some advice...

04 December 2016 3,358 7 View

Yeast inhibited by acetone at 3.25% - used to be inhibited at 12.5%?

I have been screening medicinal plants against Cryptococcus neoformans for approximately 1 year now. For about 9 months the H99 strain I have been using has been inhibited in my control column at...

21 November 2016 5,437 5 View

Resuspending plant methanol extracts in acetone for MIC assays against yeast

I am using a protocol to screen plant extracts against yeast C. neoformans. The protocol states that extracts should be resuspended in acetone to 10 mg/mL because bacteria and yeast can mostly...

02 July 2016 4,819 3 View

I have a pelargonium (P. betulinum) which is not producing its characteristic camphor scent? Any reasons why this might be so?

I am about to screen this plant for activity against the yeast Cryptococcus neoformans. What could the possible reasons be for the non-expression of this terpenoid (camphor) as I am unaware of...

11 March 2016 9,346 3 View