Samsher Singh

10 Questions 48 Answers 0 Followers

Questions related from Samsher Singh

Role of efflux pumps in virulence ?

Are there any published reports about relationship between efflux pump and secretion of virulence factors for Staphylococcus aureus or E. coli ?

04 October 2017 7,176 4 View

Can P-gp inhibitor be used to enhance the rifampicin bio-availability ?

P-gp inhibitors enhances the drug level inside cells by inhibiting the efflux. My specific question is whether a P-gp inhibitor can be used to enhance the level of rifmapicin in lungs and...

31 August 2017 2,625 3 View

Why cytotoxicity for antimicrobial compounds is assessed on HepG2/J774 cell lines knowing the fact originated from cancer cells ?

Dear researchers I wish to knew the reason why researchers use cancer cell lines for assessing the cytotoxicity. Do they mimic the normal cells of human ? If yes then how ?

30 December 2016 1,538 4 View

Is there any software to make figures for membrane transport through transport proteins like efflux/influx of drugs ?

Dear Researchers I have came across some softwares which are helpful in drawing the immunological pathways, metabolic pathways but I cant find the software which can be helpful to draw the simple...

01 October 2016 7,242 0 View

Quantity of EtBr to be added in Agarose gel for RNA ?

I am totally confused about the RNA agarose gel. I have isolated total RNA from S. aureus. I want to see it in agarose gel. I ran 1% gel with formaldehyde and added 1 ug/ml EtBr during casting....

26 August 2016 8,056 7 View

Is it worth to treat persister cell population by giving 50X or 100X MIC concentration which is not clinically achievable ?

If we have to treat some biofilm or persistent cell population then why researchers are trying to test their killing concentrations which are far beyond the clinical achievable level. e.g. For...

26 May 2016 3,385 3 View

Can a biologist think of pure demarcation between vegetarian or non vegetarian ?

Is there a pure line demarcation between vegetarian and non-vegetarian ? If somebody talks of purely vegetarian, is he not eating yeast, bacteria, molds? Need only scientific input.

11 January 2016 655 2 View

Does anybody know of any drug targets for Latent Tuberculosis?

I wish to know the latent tuberculosis models which could mimic the natural mode of infection.

05 January 2016 5,235 9 View

How to convert FLU in Arbitrary Units for fluorescence?

I am measuring the fluorescence of Ethidium Bromide during the efflux process using various bacterial strains. Its total uptake is different in different organism but I need to compare all. So I...

26 June 2015 3,592 2 View

Reactive oxygen species in M tuberculosis under hypoxia ?

I came across the two contradicting views that ROS levels must be low under low oxygen (microaerophillic) or in absence of oxygen (hypoxia). Other researcher shows that ROS levels are high due to...

01 January 1970 8,992 2 View