Can anyone recommend an antibody to use on Western blot as a loading control for S. cerevisiae cell extracts?

We need to analyze budding yeast cell extracts by Western blotting, and are looking for a good antibody against a "housekeeping" protein, serving as loading control (actin, tubulin, Gdh, etc.)....

02 March 2014 6,448 12 View

How to use the time information stored in the frames of a video in ImageJ instead of individually labeling the stacks?

I have a microscopy video (frames as stacks in a .tiff) which was acquired using a times series consisting of: 13 frames from time 0 to time 2:00, 1 frame taken every 10 s, 12 frames until 12:00...

03 April 2013 302 15 View

Hydration shell of amino acids and ions?

Does anybody know where I can find information on hydration shells of different biological ions, like free amino acids and ions (Na+, K+, Ca2+, Mg2+)? Thanks.

02 March 2013 6,314 6 View

Ubiquitination in S. cerevisiae - recommended antibodies?

In my lab we are trying to detect ubiquitination on a membrane protein in yeast. We can see bands corresponding to the size of the mono-, di- and tri-ubiquitinylated form of the protein on Western...

31 December 2012 6,347 10 View

Is there a tool to find the exact phosphosite position when phosphopeptides from MS are mapped to proteins?

I am analyzing MS phosphoproteomics data from S. cerevisiae. I have a list of peptides matched to proteins, and now I need to know 1. where my phosphosite resides in the protein (position) 2....

11 December 2012 6,080 12 View

How to import data from EXCEL tables into LaTeX?

I am currently writing my PhD thesis using LaTeX, and I'm wondering whether anybody has experience with inserting or importing large multicolumn data from .xls-files into a .tex-file. I am...

09 October 2012 3,231 21 View

Plasmid extraction from yeast - can the plasmid be altered?

I isolated a plasmid (pRS vector) from yeast using a zymolase-based method. The cerevisiae strain was a deletion strain supplemented with the plasmid copy of the gene. (Unfortunately, I didn't...

09 October 2012 2,141 14 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View