I am looking for numbers of amino acid abundance in the total proteins of different species, e.g. E.coli 3% Ala, 5 % Gly...; S.cerevisiae 5% Lys, 2.5% Pro...
Are there online databases or papers about this?
Hello Maria,
you will need an additional step to convert codons to amino aids, but in this database you can find the codon usage for a lot of micro-organisms:
http://www.kazusa.or.jp/codon/
Using this information you should be able to calculate the percentages you're looking for.
Thanks Christian, exactly what I was looking for.
We need to analyze budding yeast cell extracts by Western blotting, and are looking for a good antibody against a "housekeeping" protein, serving as loading control (actin, tubulin, Gdh, etc.)....
02 March 2014 6,548 12 View
I have a microscopy video (frames as stacks in a .tiff) which was acquired using a times series consisting of: 13 frames from time 0 to time 2:00, 1 frame taken every 10 s, 12 frames until 12:00...
03 April 2013 403 15 View
Does anybody know where I can find information on hydration shells of different biological ions, like free amino acids and ions (Na+, K+, Ca2+, Mg2+)? Thanks.
02 March 2013 6,418 6 View
In my lab we are trying to detect ubiquitination on a membrane protein in yeast. We can see bands corresponding to the size of the mono-, di- and tri-ubiquitinylated form of the protein on Western...
31 December 2012 6,435 10 View
I am analyzing MS phosphoproteomics data from S. cerevisiae. I have a list of peptides matched to proteins, and now I need to know 1. where my phosphosite resides in the protein (position) 2....
11 December 2012 6,161 12 View
I am currently writing my PhD thesis using LaTeX, and I'm wondering whether anybody has experience with inserting or importing large multicolumn data from .xls-files into a .tex-file. I am...
09 October 2012 3,523 21 View
I isolated a plasmid (pRS vector) from yeast using a zymolase-based method. The cerevisiae strain was a deletion strain supplemented with the plasmid copy of the gene. (Unfortunately, I didn't...
09 October 2012 2,251 14 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View