Guus B Erkens

2 Questions 50 Answers 0 Followers

Questions related from Guus B Erkens

How would you go about measuring high-affinity binding?

I have worked on a protein that binds its ligand with a sub-nanomolar dissociation constant. By monitoring the quenching of the intrinsic tryptophan fluorescence, I could assay substrate binding...

04 April 2013 6,552 18 View

Can anyone suggest a good protocol for the labeling of a 2-log DNA ladder with 32P radioactive nucleotides?

When I am comparing my 32P nucleotide-labelled DNA sample with a 32P end-labelled maker on agarose gel electrophoresis, the radioactive signal from the maker is much lower. Is there a way to label...

02 February 2013 4,469 9 View