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Questions related from Maria Rompf
We need to analyze budding yeast cell extracts by Western blotting, and are looking for a good antibody against a "housekeeping" protein, serving as loading control (actin, tubulin, Gdh, etc.)....
03 March 2014 6,515 12 View
I am looking for numbers of amino acid abundance in the total proteins of different species, e.g. E.coli 3% Ala, 5 % Gly...; S.cerevisiae 5% Lys, 2.5% Pro... Are there online databases or papers...
07 July 2013 5,340 2 View
I have a microscopy video (frames as stacks in a .tiff) which was acquired using a times series consisting of: 13 frames from time 0 to time 2:00, 1 frame taken every 10 s, 12 frames until 12:00...
04 April 2013 366 15 View
Does anybody know where I can find information on hydration shells of different biological ions, like free amino acids and ions (Na+, K+, Ca2+, Mg2+)? Thanks.
03 March 2013 6,386 6 View
In my lab we are trying to detect ubiquitination on a membrane protein in yeast. We can see bands corresponding to the size of the mono-, di- and tri-ubiquitinylated form of the protein on Western...
01 January 2013 6,410 10 View
I am analyzing MS phosphoproteomics data from S. cerevisiae. I have a list of peptides matched to proteins, and now I need to know 1. where my phosphosite resides in the protein (position) 2....
12 December 2012 6,137 12 View
I isolated a plasmid (pRS vector) from yeast using a zymolase-based method. The cerevisiae strain was a deletion strain supplemented with the plasmid copy of the gene. (Unfortunately, I didn't...
10 October 2012 2,200 14 View
I am currently writing my PhD thesis using LaTeX, and I'm wondering whether anybody has experience with inserting or importing large multicolumn data from .xls-files into a .tex-file. I am...
10 October 2012 3,426 21 View
