I am interested in parallel genomic and transcriptomic sequencing at the single cell level but with the high throughput capacity of a system like 10X. I understand that this is doable at a low-throughput level via techniques like SMARTseq2, but I am wondering if such an option exists for HT methods like 10X, DropSeq, etc.
Thanks!
So far I have not seen a platform that can do HT scDNA-seq. The challenge is that unlike RNA molecules that are in large numbers, each diploid cell has only two copies of genomic DNA (one copy from each parent). LT approaches rely on whole genome amplification, which still misses a lot of genomic regions due to technical factors such as stochastic effects, preferential amplification of some regions over others, etc.
Dear Wang, . I am Kiran work as Product and application manager for NGS portfolio in DSS Takara Bio. Takara has one automated solution known as ICELL8 cx Single-Cell System.Please do feel free to reach for any further information is required.
Hi Eugene,
I'm currently in the process of optimizing and planning a pilot experiment using the DNTR method explained below:
"A Highly Scalable Method for Joint Whole-Genome Sequencing and Gene-Expression Profiling of Single Cells": https://www.sciencedirect.com/science/article/pii/S1097276520306559
Let me know if you like to brainstorm together.
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