Hello! I am looking to isolate cell nuclei from mouse brains (hypothalamus specifically) and have been considering several kits. There are two from Sigma - the Nuclei EZ Prep and Nuclei Pure Prep, as well as the Minute Single Nucleus isolation kit - offered both with and without detergent.
I have considered adapting a 'home-brew' protocol described in some recent papers, but due to time constraints, a kit would be ideal since there might be less troubleshooting and validation.
Does anyone have experience with these kits and do you have a recommendation as to which might work best?
Hi Eugene, I was thinking the same after my own various in-house variations. I have used the Nuclei EZ kit, which is actually just a detergent-containing buffer, and not a true kit. I did get good results with it but I was using it as part of a Fluorescence- Associated Nuclei Sorting protocol, so I was always guaranteed pure nuclei. I do have my eye on the Minute Single Nucleus products, so if anyone else has used these?
Thanks,
RT
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies