We work on oral cancer inhibition by plant phytoconstitution. We found one of the plant phytocompound working very effectively against different cancer proteins on western blots experiments. Now we have submitted the work to a journal and the reviewer asked about the positive control. This is missing in our study. I’m rather confused concerning the necessity and application of a positive control during this study design. Could anyone provide more advice or suggestions during this case?
I fully share the opinion of Erdogan.
If you trust the RG community scientists, you should indicate us a little bit more about your "phytochemicals" AND plant AND proteins and we can therefore certainly help you in choosing a positive control.
An "universal" positive control does not exist.
Robert
PS: I will not publish your data if you share with us some "confidential" infos ...
It all depends on the proteins you are looking at post-treatments. In order to provide you with positive controls, you need to indicate which proteins you are looking at.
Thanks Dr. Patrick Aouad we done pretreatment work TGF beta is our protein
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