Hello Dear scientists, Please would you suggest me an idea on bacterial species identification to genus level, hence due to some financial shortage, i couldn't proceed to bacterial sequencing due to lack of automate DNA sequencer, please would you recommend me the simplest techniques. Thank for your understand and support!!!!!!
Hi..
There are several techniques that can be used to identify bacterial species to the genus level, even if you do not have access to an automated DNA sequencer. Here are a few options you might consider:
I hope these suggestions are helpful!
If you are interested in a precise taxonomy classification, 16S rRNA gene is still the efficient approach for that. It is used to reduce the burden charge of NGS. You can use the short-read sequencing platform or even better the longest one.
Depends on your budget (and origin of bacteria), but there are commercial kits to identify most common human pathogens by colorimetric & culturing methods.
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