Collagen coating

Does ECM affects cell growth or other properties? Could lacking of ECM or disruption of its structure alter the cell biology?

08 September 2012 7,942 2 View

ELISA of tongue

Has anyone ever extracted protein from tongue tissue to perform ELISA? What kind of lysis buffer did you use?

07 August 2012 6,445 4 View

Quantification of Ki67

Does anyone know how to quantify the result of Ki-67 labelling?

06 July 2012 3,366 23 View

Mouse primer

Can anyone provide me good primers of TNFa, IL-10, IL-5, IL-13, RANTES, IL-17A for Sybr green qPCR use?

05 June 2012 5,617 4 View

Mast Cell Depletion

Does anyone know how to deplete mast cell in vivo? Is there any known antibody can serve this purpose?

05 June 2012 2,791 16 View

Fibrosis detection

How can I detect fibrosis within a tissue? Any staining to apply on biopsy or other methods?

05 June 2012 1,781 15 View

Cd34

In which cells is CD34 expressed? What's its function?

04 May 2012 1,000 13 View

COX 2 (cyclooxygenase 2) primer

Have anyone used the primer for qPCR to detect cyclooxygenase 2? May I learn the sequence of them??

01 February 2012 2,715 4 View

NCBI : This record was replaced with Gene ID: 17386

I was looking for the primer of "MMP1" of mouse, yet it shows the message as the title and redirect me to the page named "MMP13", does it means they are the same in mouse?

01 February 2012 7,028 3 View

systemic infection caused by Somonella spp?

Dose Somonella spp. often cause systemic infection like bacteremia or spleen invasion?? how does that happen???

31 December 2011 1,842 1 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Can you recommend a protocol for immunohistochemistry of mouse brain sections using anti-MAP2?

Current protocol does not give clear images.

04 January 2021 1,614 3 View

I need to know about immunohistochemistry steps?

Immunohistochemistry staining. Tissue Fixation during IHC. Using DAB. blocking step. controls group.

23 December 2020 5,768 2 View

ISO antibody against BFP for FFPE IHC?

Suggestions and recommendations welcome. Any usage/protocol experience would also be helpful/appreciated. Cheers

22 December 2020 599 3 View