ELISA of tongue

Has anyone ever extracted protein from tongue tissue to perform ELISA? What kind of lysis buffer did you use?

07 August 2012 6,445 4 View

Quantification of Ki67

Does anyone know how to quantify the result of Ki-67 labelling?

06 July 2012 3,366 23 View

Mouse primer

Can anyone provide me good primers of TNFa, IL-10, IL-5, IL-13, RANTES, IL-17A for Sybr green qPCR use?

05 June 2012 5,617 4 View

Mast Cell Depletion

Does anyone know how to deplete mast cell in vivo? Is there any known antibody can serve this purpose?

05 June 2012 2,791 16 View

Fibrosis detection

How can I detect fibrosis within a tissue? Any staining to apply on biopsy or other methods?

05 June 2012 1,781 15 View

Regenerating marker

To assess the recovery of tongue epithelial cell, what are the marker to look into? Ki67?

04 May 2012 9,093 4 View

Cd34

In which cells is CD34 expressed? What's its function?

04 May 2012 1,000 13 View

COX 2 (cyclooxygenase 2) primer

Have anyone used the primer for qPCR to detect cyclooxygenase 2? May I learn the sequence of them??

01 February 2012 2,715 4 View

NCBI : This record was replaced with Gene ID: 17386

I was looking for the primer of "MMP1" of mouse, yet it shows the message as the title and redirect me to the page named "MMP13", does it means they are the same in mouse?

01 February 2012 7,028 3 View

systemic infection caused by Somonella spp?

Dose Somonella spp. often cause systemic infection like bacteremia or spleen invasion?? how does that happen???

31 December 2011 1,842 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View