27 Questions 24 Answers 0 Followers
Questions related from Patrick Kao
Does ECM affects cell growth or other properties? Could lacking of ECM or disruption of its structure alter the cell biology?
09 September 2012 8,005 2 View
Has anyone ever extracted protein from tongue tissue to perform ELISA? What kind of lysis buffer did you use?
08 August 2012 6,500 4 View
Does anyone know how to quantify the result of Ki-67 labelling?
07 July 2012 3,428 23 View
Can anyone provide me good primers of TNFa, IL-10, IL-5, IL-13, RANTES, IL-17A for Sybr green qPCR use?
06 June 2012 5,680 4 View
How can I detect fibrosis within a tissue? Any staining to apply on biopsy or other methods?
06 June 2012 1,841 15 View
Does anyone know how to deplete mast cell in vivo? Is there any known antibody can serve this purpose?
06 June 2012 2,851 16 View
To assess the recovery of tongue epithelial cell, what are the marker to look into? Ki67?
05 May 2012 9,146 4 View
In which cells is CD34 expressed? What's its function?
05 May 2012 1,067 13 View
I was looking for the primer of "MMP1" of mouse, yet it shows the message as the title and redirect me to the page named "MMP13", does it means they are the same in mouse?
02 February 2012 7,082 3 View
Have anyone used the primer for qPCR to detect cyclooxygenase 2? May I learn the sequence of them??
02 February 2012 2,780 4 View
Dose Somonella spp. often cause systemic infection like bacteremia or spleen invasion?? how does that happen???
01 January 2012 1,901 1 View
Can anyone tell me what it is??
01 January 2012 4,405 0 View
Is there any known way to inhibit innate immunity??
12 December 2011 2,898 16 View
I saw a term GL7 appears in a paper. What is it? What does it mark??
11 November 2011 240 1 View
What markers would you use to gate GC B cells from follicle B cells??
11 November 2011 1,824 4 View
What is Mxl protein? In what kind of cells does it express? in a Mxl-Cre system, when and in which cell would Cre function?
11 November 2011 4,546 0 View
What are they?? Are they specific for something??
11 November 2011 679 0 View
Both are lymph organs, what are the characters different from each other??
11 November 2011 4,395 1 View
Is there a website or sth that I can find primer sequence of a specific protein?? like if I'm looking for primer for IL-33 of mice, where can I find it??
11 November 2011 3,299 1 View
What are the principles or precautions for primer designing?? Which are the criterias for a primer to be a good primer??
10 October 2011 570 6 View
as title. B cell, unlike T cell, doesn't have helper form or cytotoxic form. So what does B cell differentiation means? Does it mean class-switching? How can I differentiate "B cell...
09 September 2011 10,059 9 View
If I want to stain neutrophil and macrophage on biopsies, which commercial kits are recommended??
09 September 2011 6,640 1 View
Is there a pH-sensitive dye which only show it's color in low pH environment??
09 September 2011 8,794 1 View
When using flourescence microscope, we found light spots in the control which we didn't add any dye, what might those be?
09 September 2011 235 0 View
What does the CD11b and CD11c mark??
08 August 2011 7,491 28 View
I'm observing the recruitment of mast cell to the region which was irradiated, but I'm not so sure which chemokines to look at, so hope can get some advise!
08 August 2011 6,916 1 View
How can I find out the details of certain strain of mouse? like: if I'm using WBB6F1, where can I get the information of it??
08 August 2011 2,762 2 View
