I have isolated recombinant plasmid using all buffer solutions from GeneJet Mini prep plasmid isolation kit except resuspension solution. I used resuspension solution of promega Midi prep kit. I got band during electrophoresis. Will this change of one solution from another company affect downstream steps of my experiment?
Hi,
tipically commercial solutions for resuspending DNA are almost quite similar between each other. They are essentially slightly alkaline solutions (pH 8) with or without a cation chelator (tipically EDTA). The effects on downstream steps are difficoult to predict without knowing witch experiments you are running. I have to advise you that a buffer containing EDTA could reduce the yield of subsequent enzymatic reactions that often need metal cations. After DNA isolation using commercial kits, I usually resuspend DNA pellet in sterile water to avoid this problem and always worked well.
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