What the best simulator for integrate bc in iot for device authentication?

I am looking for a simulator to IOT device authentication based on blockchain without using physical device

01 February 2021 167 3 View

Greenhouse Simulation using COMSOL Multiphysics ?

Have anybody tried to simulate greenhouses using COMSOL Multiphysics? and is it doable?

26 December 2020 803 1 View

Did anyone used microflow imaging to measure the size distribution of Multivesicular liposome which are charcterized with size (>3um)??

I have prepared Multivesicular liposomes using the following procedures: Multivesicular liposome formulations for the sustained deliv... Multivesicular liposome (MVL) sustained delivery of a...

18 November 2020 3,189 2 View

CROs or collaborators for assay of non-ATP competitive kinase inhibitors?

We have compounds that showed activities against variety of kinases. However, since they have been designed to bind to the peptide site on kinases, I need to investigate this mechanism. Please...

15 November 2020 2,040 3 View

Are there sources benefit from the sacrifice of children in ancient Greece?

I knew an exciting ritual in Greece, including the sacrifice of children, especially maimed them. Is there sources or studies on this topic .. Thank you for your help

14 October 2020 2,082 4 View

I followed a common procedure to synthesize Fe3O4 nanoparticles. The problem is that XRD pattern does not show any crystal structure. Could you help?

Besides, I have performed different characterization tecniques to my Fe3O4 nanoparticles and all are similar to what is reported in literature. Those characterization techniques include FTIR, SAED...

22 July 2020 8,549 12 View

Please recommend biological investigation of newly identified anticancer compounds that specifically inhibit Src kinase?

We aim to publish ta high impact journals (If 6+). Compounds showed incredible activities on cancer cell line (submicromolar on several types). I need your recommendation on how to determine...

24 May 2020 1,230 3 View

To what extent do top-down language policies influence language use?

I'm often very skeptical about the language decisions and policies issued by governments or self-proclaimed language authorities to control the way people use language. Nevertheless, I'm just...

28 February 2020 5,904 4 View

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19 September 2019 5,877 1 View

Will a power fail affect my cDNA synthesis?

14 September 2019 8,969 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View