Cleaning up single cell RNA from 10x dataset?

Hello, I am new to single cell RNA data processing and am currently running my data through the initial cleanup steps via Seurat on R. I have previously done this on epithelial organoid data and...

23 December 2020 4,709 1 View

Fluorimeter giving odd results for carbon quantum dots?

I made a Pb sensor using carbon quantum dots (CQDs). I add buffer, CQDs, and a given metal to a quartz cuvette of the recommended type. Before adding a metal, the cuvette will emit a yellow-green...

16 December 2020 9,675 3 View

How much salt in a m^3 of water would be needed to prevent evaporation ?

Assume you have 1 cubic meter of water with 1 square meter of surface exposed to the sun. If you kept adding salt to the solution, is there a point at which evaporation would cease? Assuming the...

22 November 2020 5,444 1 View

Automatic cell counting? (Ductal epithelium cells with fluorescence)?

Hello, I am currently conducting a lineage tracing experiment where I need to count GFP+ cells in multiple compartments of ductal epithelium (frozen tissue sections of an inducible Cre mouse with...

20 September 2020 1,855 6 View

Resuspension mutant huntington (mHTT) in conditioned medium?

Hello all. In lab we grow cells to secret mHTT in media that we then collect and subject to centrigufation at 100k G for 2 hours to concentrate the protein and other secreted components of the...

30 August 2020 1,858 1 View

How to improve my CBD to THC conversion method?

Hello Everyone, Let me preface this question by saying that this is all being done under a legal THC/Hemp license in the state of Colorado. I was hoping to get some advice on how to improve my...

27 April 2020 6,292 1 View

Is there literature on pre-steady state "lag" for oxidase enzymes?

Greetings, I'm looking for literature related to a topic I want to write a manuscript on. I'm working with an glycine oxidase enzyme and noticed when I monitor the rate via spectrophotometry and a...

31 March 2020 3,917 2 View

What's going on with my HPLC?

Hi Everyone, I was wondering if you guys had any ideas/suggestions that I can work with to try to fix my HPLC. I have attached a picture of one of our Blanks (100% ACN). This shape is unusual,...

16 March 2020 6,454 5 View

Does anyone have any literature showing 'political or civil engagement' as an important factor for refugees when deciding on repatriation?

This is for research about important issues to repatriation of refugees. Literature (or UNHCR documents) connecting forced migration/repatriation to political/civic engagement would be...

04 March 2020 6,616 3 View

How to BLAST thousands of microbiome sequences?

I'm an undergraduate student completing a capstone project working to characterize a plant microbiome. I have a couple thousand fungal ITS sequences in a fasta file. Is there any way to analyze...

03 November 2019 6,622 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View