Recombinant protein purification, fail to elute the GST tagged protein using a reduced glutathione buffer?

My GST tagged, recombinant ，target protein of 10 kDa， expressed in BL21, was failed to be eluted in a 16mM of reduced glutathione buffer。However， I find my protein down，binded with the GST bind...

19 October 2020 7,182 3 View