I have several proteins that I wish to express in yeast. I have some funding to contract out this work. Does anyone know of a company they could recommend, either in this forum or a private message. I have used two companies and I am not happy with either.
Thanks!
Dear Felix,
The protein expression facility at The University of Queensland, in Australia has amazing facility for expression of proteins in bacteria, yeast, insect cells and mammalian cells. You can contact Associate Professor Linda Lua, who is the director of the facility. Here is her profile on researchgate.
https://www.researchgate.net/profile/Linda_Lua
Thanks Alemu! I will consider your suggestion. I am in the United States and importation of organisms produced outside the country can be a problem, though perhaps UQ has ways of smoothing shipments.
Dear Felix,
According to my experience, yeast expression is a good system, you can choose S. cereviseae system with pYES/CT plasmid with His tag.You can go for Pichia system as well. The advantage of Pichia expression is you allow yeast to secrete and purify the recombinant protein from culture supernatant. If you are working on Eukaryotic proteins which require any post translational modification, I would go for the yeast expression system. Good luck
Thanks Thamaral,
We have had only about 30% success rate with expression in Pichia. We need to use a contract lab, as I do not have staff that I can assign for this project. I was hoping some folks would have a suggestion for a good lab to do these recombinant protein expressions for us.
- Badges
- Science method
- Similar topics
- Biological Science
- Genetics
- Gene Expression
- Protein Expression