01 January 2020 2 6K Report

I overexpressed soluble cytochrome c in e.coli. After Ni-NTA. I am getting two protein bands on SDS-PAGE - the one which matches my theoretical protein molecular weight 40 kda is having a fainter band(upper band) than the other band which is 33kda(lower band)? With the mass spec analysis , both bands contains the similar number of peptides from the same protein.

What could be the possible reasons?

Thankyou

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