Recently, I used an abcam ELISA kit for estradiol (ab108667). All the results were below the curve because apparently it had to put a blank, however it was not specified in the instructions. Has anyone used this kit? How could "calculate" the results of Abs. that did I get?
Dear Cinthia Juarez-Tapia!
Here are the ways to calculate the results of Absorption:
13.CALCULATIONS
Calculate the mean background subtracted absorbance for each point
of the standard curve and each sample. Plot the mean value of
absorbance of the standards against concentration. Draw the best-fit
curve through the plotted points. (e. g.: Four Parameter Logistic).
Interpolate the values of the samples on the standard curve to obtain
the corresponding values of the concentrations expressed in pg/mL.
In addition, you will get the whole booklet for ab108667
Sincerely Yours!
Dr. Bratko Filipič
E-mail: [email protected]
Did you consider that this ELISA kit is intended for the use in serum or plasma? Any other matrix has to be validated carefully.
Hi, I used it twice and I obtained good results.
In the blank you don´t have to put the ESTRADIOL-HRP Conjugate, you add nothing. Because the conjugate is going to bind to the capture antibody and will give you a signal. You only wash and then add the TMB substrate for all the wells and follow the protocol.
The concentration of 17-beta-estradil will depends in wich day of the pregnancy you take the sample. Because I was not sure to measure something, I concentrated my samples with amicon filter of 10 kDa molecular cutoff.
Write me if you have more questions (I speak spanish).
Best,
Carolina
Hello Cinthia Juárez-Tapia,
Creative Diagnostics offers multiple assay formats including traditional sandwich ELISA kits, competitive ELISA kits, as well as cell-based ELISA Kits for many targets. Maybe you can have a try.
https://www.creative-diagnostics.com/symbolsearch_Estradiol.htm
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