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Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...
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Hello everyone, Could someone please recommend me a secondary biotinylated antibody that does not bind to the plate inespecifically giving high background noise reaction? I appreciate your help...
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Hey everyone, For those that perform "homemade" ELISA, I have been titrating the different components of a sandwich ELISA. The most "acceptable" curve that I have obtained reaches an absorbance...
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Hey Everyone, For the people with experience in biochemistry/ELISA. what do you do in order to prepare human blood to see measure a protein through ELISA that is not highly concentrated in...
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Hello, has someone done chessboard or checkerboard experiments for an indirect sandwich ELISA? Is it possible to titrate every antibody as it were used for an indirect ELISA??, I mean adding the...
03 March 2018 5,346 6 View
Hy everyone, Someone of you knows a good alternative for Iba1 rabbit antibody from WAKO company. The company has had problems with the production of the antibody and they are going to deliver it...
09 September 2017 680 17 View
Hy anyone, I am currently designing a home made sandwich ELISA for a PSD protein and I am facing the problem that in spite of having a nice standard curve with a recombinat-GFP protein, I achieve...
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Hi everyone, Could you please suggest me a Journal for publishing a paper that describe the establishment of a sandwich ELISA (TECHNICAL) but at the same time use that tool to measure the...
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