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Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...
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Hello everyone, Could someone please recommend me a secondary biotinylated antibody that does not bind to the plate inespecifically giving high background noise reaction? I appreciate your help...
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Hey everyone, For those that perform "homemade" ELISA, I have been titrating the different components of a sandwich ELISA. The most "acceptable" curve that I have obtained reaches an absorbance...
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Hey Everyone, For the people with experience in biochemistry/ELISA. what do you do in order to prepare human blood to see measure a protein through ELISA that is not highly concentrated in...
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Hello, has someone done chessboard or checkerboard experiments for an indirect sandwich ELISA? Is it possible to titrate every antibody as it were used for an indirect ELISA??, I mean adding the...
03 March 2018 5,348 6 View
Hy everyone, Someone of you knows a good alternative for Iba1 rabbit antibody from WAKO company. The company has had problems with the production of the antibody and they are going to deliver it...
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Hy anyone, I am currently designing a home made sandwich ELISA for a PSD protein and I am facing the problem that in spite of having a nice standard curve with a recombinat-GFP protein, I achieve...
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Hi everyone, Could you please suggest me a Journal for publishing a paper that describe the establishment of a sandwich ELISA (TECHNICAL) but at the same time use that tool to measure the...
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