In my research , i am working a lot in qRT-PCR , for more than 25 gene , in human genome , and i am in the step of primers optimization , getting the right melt curve , right CT value .. etc ,
almost all the primers worked well except some of them , i would like to know the reasons of this problem ,, to solve this problem ,
Mahdi Saber Al-Deresawi
you can do the following:
-check up the quality and quantity
-the concentration of all samples should be equal
-You can use the gradient PCR
good luck
0 votes
0 thanks
- Badges
- Science method
More Malak Nabeel Alhajahjeh's questions See All
Similar questions and discussions