Circular RNA Isolation from multiple source and qPCR with divergent primers ?

Malak Nabeel Alhajahjeh @Malak-Alhajahjeh

16 January 2021 0 7K Report

Any recommended kits used to isolate circular RNA form serum, exosomes, cells, tissue, what is the best source to isolate from ?

As its recommended for cir RNA to amplify is divergent primers, any recommendation of how should design the best primers for circular RNA, best brand, with best kit for qPCR.

Any workflow for this type of small RNA ?

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Malak Nabeel Alhajahjeh's questions See All

Does anyone know of a reliable source for primary antibodies against oxytocin and vasopressin for IHC studies in rats?

The rats are perfused with PBS and the brain sections are 18 microns thick and fixed on slides.

09 November 2020 833 3 View

Reasons of do not having good peak , right CT Value , right melt curve in qRT-PCR , while Primer optimization ?

In my research , i am working a lot in qRT-PCR , for more than 25 gene , in human genome , and i am in the step of primers optimization , getting the right melt curve , right CT value .. etc...

08 March 2019 2,724 3 View

What is the best method for serum exosome extraction?

I want to isolate serum exosomes to study their miRNA. What is the best method for serum exosome isolation? Can you use the invetrogen kit or exoquick or the classical method for exosome...

19 April 2014 454 7 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View