Hi all, I ran ddPCR and observed this 'cut off' for my 2D amplitude plot for all my samples. I have shown the non template control droplet image. In addition, all my droplets varied by a lot between samples (ranges from 3000-6000 total droplets).

Apparently the user before me covered their plate with 2 plate covers, and might have caused a hemorrhage in the droplet reader machine.

Could I ask for opinions about this? Could it really be a clogged droplet reader machine, or might there have been other problems causing this?

Thank you!