11 Questions 2 Answers 0 Followers
Questions related from Kay Leen
Hello! I am running an experiment involving biotin labelled DNA oligonucleotides. I hope to identify nuclear proteins bound specifically to my ~70bp oligonucleotides and elute the nuclear proteins...
18 April 2023 3,776 2 View
Hello! I am trying to find SNPs which are in linkage disequilibrium with my target SNP on LDproxy (https://ldlink.nci.nih.gov/?tab=ldproxy), however I realize I get very different results using...
05 October 2022 4,980 1 View
Hi all, I have a few bigwig ChIP-Seq files downloaded from ENCODE and visualized on IGV. These are histone H3K4me3 and H3K27Ac ChIP-Seq data. However, I loaded the respective narrow bed and...
13 July 2022 3,701 0 View
Hello, I am analyzing some whole genome data (I am very novice at it), and I have a list of SNPs with this format SNP_A- : SNP_A-4270094 SNP_A-8282305 SNP_A-8282312 SNP_A-8282315...
07 July 2022 3,824 0 View
Hello, I have quite a strange question to ask regarding RNA Sequencing differentially expressed list... I have two DE comparisons: (1) Disease line with deleted SNP vs disease line without...
06 July 2022 586 1 View
Hello! I wish to perform prime editing from A->G (upper strand) on induced pluripotent stem cells. However, as the 5' of my target editing region does not have PAM sites, would the figure I have...
20 February 2022 2,693 0 View
Hello! I am interested in looking at a gene (ENSG00000111863) in human protein atlas. Unfortunately, although it has high specificity in HUVEC and TIME (endothelial cell lines), it seemed to have...
09 February 2022 4,686 0 View
Hello! I would like to visualize chip-seq bigwig output files on igv and compare the histone peaks directly, however I realise it is very difficult to compare them directly due to differences in...
24 August 2021 8,951 1 View
Hi all, I ran ddPCR and observed this 'cut off' for my 2D amplitude plot for all my samples. I have shown the non template control droplet image. In addition, all my droplets varied by a lot...
23 July 2021 4,108 1 View
Hi! I'm currently running 3C for an inter chromosomal interaction, and I have a few questions about the BAC control. Is it right if I purchase 2 BAC covering my regions of interest (on chromosome...
01 June 2021 4,548 4 View
Hello! I have read many 3C papers which have emphasised that the 3C technique can be used to validate long ranging interactions for both cis and trans chromosomal interactions. However, I have...
01 January 1970 1,707 1 View
