Plasmid purification -too much isopranolol in endotoxin wash buffer?

05 December 2023 1 9K Report

We were making up the buffers for our Promega PureYield plasmid midi-prepkit and accidentally added 315ml of isopranolol to the endotoxin wash buffer instead of 210ml. Do you think we can use it?

Alessandro Paparella

wash two times more

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