Real-time immuno-PCR

Omar J BenMarzouk-Hidalgo @Omar_BenMarzouk-Hidalgo

05 May 2012 0 4K Report

of mature and inmature proteins for knowing virus activity and infectivity. I have come along different

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Science method

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Why, when performing a dynamic analysis of various materials one inside another in Abaqus, cannot evidence propagation of von misses stress?

I have a dynamic analysis with a model that has 4 layers one inside the other in 3d solid and when simulating the von misses stress there is only data from the first two layers, why can this...

29 May 2023 7,137 1 View

Functional Assays to measure interferon ?

Hi all, I am working with Leishmania (V) panamensis and in our transcriptomic analysis we saw that IFN is upregulated in an specific group of parasite strains. Our next step is to set up a...

15 May 2023 5,600 1 View

How to extract immunoglobulins from tablets?

What solvent and method is best for extraction of antibodies from tables?, I need to quantify IgG1 by radial immunodiffusion technique and I'm considering to use 20% methanol in PBS (pH 6.5...

02 October 2022 3,705 0 View

Does anyone have ever used DETAnonoate?

Dear all, We are trying to standarize an assay to detect NO production in macrophages and we are planning to buy DETAnonoate; however, the papers that I have read to not specify much how to...

09 May 2022 4,107 0 View

How to avoid the differentiation into cholangiocytes from hepatoblast in the development of hepatic organoids?

I am working on the development of hepatic organoids from iPSC. I am looking for in the bibliography but I cannot find any protocol on how to avoid the differentiation of the hepatoblast into...

07 March 2022 9,580 0 View

Which of these qualitative analysis software NVivo or ATLAS.ti is better for working with scientific contributions type articles?

I would like to have a more formed judgment criterion to decide on one of these two qualitative information analysis tools. Please, I would greatly appreciate your advice and suggestions. Thanks.

06 May 2021 3,137 8 View

How to interpret hierarchical regression when a predictor is no longer significant after new variables are added?

Hello everyone: What does it mean when a variable is significant in one step but then in the next step it's no longer significant? For example, in this article...

29 November 2020 7,732 3 View

UV-Vis of Toluene. Having absorption values after doing a blank with the same compound?

I am preparing a solution of 0.02 mmol of compound A in 2ml of acetonitrile or toluene. The solution of compound A in acetonitrile is then measured by UV-Vis (600 to 190 nm range) using a high...

20 November 2019 6,286 2 View

Dear Cui, congratulation for this amazing manuscript. I was wandering if you could specify the protocol of transference used in WB,?

Dear Cui, your images of WB are very nice and since I am working in a project that involved mTOR in reproduction and I can´t get my WB works, I was wondering if you can share the protocol of...

03 March 2019 6,178 0 View

What solvent can I use to dissolve an organic-inorganic hybrid to perform liquid 31P-NMR?

I can´t manage to dissolve a solid Nitrogen-containing heterocycle-Polyoxometalate hybrid material to perform a liquid 31P nmr. I´ve tried: Solvent relative polarity CHCl3...

01 May 2018 3,768 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

How do I run a dead well test for a QuantStudio 5 qPCR machine?

I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...

24 July 2024 8,172 1 View