How to interpret hierarchical regression when a predictor is no longer significant after new variables are added?

Hello everyone: What does it mean when a variable is significant in one step but then in the next step it's no longer significant? For example, in this article...

29 November 2020 7,354 3 View

UV-Vis of Toluene. Having absorption values after doing a blank with the same compound?

I am preparing a solution of 0.02 mmol of compound A in 2ml of acetonitrile or toluene. The solution of compound A in acetonitrile is then measured by UV-Vis (600 to 190 nm range) using a high...

20 November 2019 6,137 2 View

What solvent can I use to dissolve an organic-inorganic hybrid to perform liquid 31P-NMR?

01 May 2018 3,659 4 View

Building a Knowledge Graph on Renewable Energy Sources?

Hello everyone: Many countries around the world are looking for updated and integrated data and information related to Renewable Energy Sources for solving practical problems in this area. The...

01 February 2018 5,152 4 View

Could burned land be recovered?

The bioremediation is used for recovery contaminated lands, but is possible recover a land burned?Bacterias that use carbonized matter? Maybe is a crazy idea...

06 February 2017 9,574 5 View

[SOLVED] How can I solve the "Cannot read FoldX RepairPDB output" error in Yasara/FoldX?

I am trying to model point mutations in protein structures in Yasara using the FoldX plugin but I always get the same error: "Cannot read FoldX RepairPDB output". This is apparently a known error...

06 July 2016 7,121 2 View

[SOLVED] How can I solve the "Cannot read FoldX RepairPDB output" error in Yasara/FoldX?

I am trying to model point mutations in protein structures in Yasara using the FoldX plugin but I always get the same error: "Cannot read FoldX RepairPDB output". This is apparently a known error...

05 July 2016 7,716 2 View

How can I peform IHC for CD3, CD4 and CD8 with frozen sections or PFA?

06 July 2015 9,410 11 View

Development of an ELISA assay?

I am trying to set up an ELISA assay for the detection of an HIV-1 protein, but up to this point no success has been achieved. The plan is to set a sandwich ELISA, I have tried several protocols...

08 September 2013 6,951 45 View

Development of an ELISA assay?

I am trying to set up an ELISA assay for the detection of an HIV-1 protein, but up to this point no success has been achieved. The plan is to set a sandwich ELISA, I have tried several protocols...

01 September 2013 6,470 42 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View