RBC intracellular calcium measurement by flow cytometry using calcium green indicator dye?

More Ebrima Danso's questions See All

Please does anyone knows how to model green phosphorene? or I would be very grateful if anyone has the cif file and would send it to me. Thank you?

I am trying to model green phosphorene for my studies but the I don't know how to go about the modeling.

12 February 2023 1,237 0 View

How do I measure Iron (Fe) in monocyte before and after culture with MTB?

Dear all great researchers, I intend to measure the level of iron concentration in monocytes before I infect them with mycobacterium tuberculosis. The idea is, I want to assess the role of...

13 January 2021 7,583 2 View

Please I need help with high pressure dft calculations of elastic properties?

I am calculating the elastic constants of a trigonal al2O3 using Castep. After geometry optimisation at 0 Gpa pressure the crystal structure changes to a cubic structure.

13 January 2020 4,495 15 View

How many grams of Yoshida nutrient powder do i need to make nutrient solution?

I am doing drought stress experiment and i want to know about the grams of yoshida powder i need to make the nutrient solution for the plant.

12 December 2019 276 1 View

Does anybody knows of a chemical company besides Gelest that makes/sells customized reactive silanes?

I am in urgent need of a monoTriEthoxysilylethyl terminated PolyDimethylSiloxane. I already tried many companies including Gelest but I could not still find it. Any information will be highly...

16 October 2016 7,641 2 View

Does any one know the best current method to measure calcium flux in RBCs?

I am interested in looking at the mechanistic effects of polymorphims in the ATP2B4 gene and malaria susceptibility.

19 April 2016 4,884 3 View

How do I calculate actual ETc with the soil water balance method on days without soil moisture measurement?

For irrigation scheduling, I used Penman Monteith equation (Allen et al, 1998) to estimate Crop evapotranspiration (ETc). However, in calculation of actual ETc, I used the soil water balance...

07 October 2015 8,448 3 View

When do ex vivo experiments cross the line and become in vitro experiments?

If I obtain splenocytes, stimulate them for few hours and measure cytokine profile within the cells afterwards, will this be considered ex vivo or in vitro? I figure this must be ex vivo, am I...

09 June 2015 7,192 4 View

How do I determine the electrical conductivity of sandy soil samples using a soil water ratio of 1:5?

I want to determine the electrical conductivity of some soil samples. I mixed 15 g soil in 75 ml water and shake for one hour followed by 30-min settling time. The electrical conductivity of the...

05 March 2015 208 3 View

Please what are the steps and procedures for these Biochar properties? Please kindly advice and assist

Dear Professors and fellow Colleagues, Please I am working with corn straw biochar in CHINA and have no previous experience with. Due to circumstances I can access Google and others which has...

01 January 1970 6,801 3 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Looking for help on sem image analysis?

Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...

01 August 2024 572 0 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View