If I obtain splenocytes, stimulate them for few hours and measure cytokine profile within the cells afterwards, will this be considered ex vivo or in vitro? I figure this must be ex vivo, am I right? What if I don't do any stimulation but decide to measure cytokine profile at base line from these cells, shouldn't this be ex vivo instead, and the former question, in vitro?
As I remmber correctly all experiments with dissected or isolated tissues are considered as "in vitro".
Yea I agree we classify (in our lab/papers) such experiments as in vitro
I would describe your experiment as being in vitro primary culture. If you worked with the whole spleen, I would call it ex vivo organ culture.
If you're treating the individual (animal/human) to stimulate the splenocytes, I'd call it ex vivo; but it sounds like you're doing that after the splenocytes come out, so I'd agree that it's in vitro.
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