Does any one know the best current method to measure calcium flux in RBCs?

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Laszlo G Meszaros

You could first try some Fura-based Ca indicators with a fluorescent microscope.

Ebrima Danso

Thank you Laszlo

To be specific, I have a calcium green indicator dye for this experiment. I intend to use BD Accuri C6 flow cytometer to measure RBC intracellular calcium concentration after loading the RBCs with the indicator dye.

Hope this is clearer and hope it generates more help.

Thanks a bunch