How much 200mg/ml solution should i add to 300ml base to get the final solution of 40ug/ml ?

I have a 200 mg/ml ampiciline solution, how much should i add to 300 ml of LB medium to get the final concentration of 40 ug/ml?? How much bacteria (in ml) should i add to 75 ml of medium to...

05 October 2020 6,449 5 View

Paper questionnaires in qualitative research?

Hello, I am looking for literature on methodological aspects of using paper questionnaires in qualitative research. Questionnaire is usually associated with quantitative approach but what with...

30 June 2019 5,442 12 View

I have experienced a strange problem with Sanger DNA sequencing, any hint?

I am trying to sequence a PCR product, the length is sufficient, it on agarose gel it gives nice, sharp band, without any smear or clouds of leftover oligos. Concentration measured on...

06 August 2018 5,025 3 View

Why magnetite nanoparticles become brown in suspesion after sonication?

20 June 2016 1,446 8 View

Can anyone suggest some scientific institutions in Australia which research area is concentrated around the phenomenon of luminescence?

I am trying to make a list of such institutions (institutes, departments, etc.) to get a general idea of the presence of this topic in Australia. So far I have managed to find the Ultrafast and...

10 July 2014 4,119 5 View

Protein extraction from human brain tissue for detection of transmembrane proteins?

I need to detect ion channel on human brain tissue extract by WB. I am wondering if I should use some special buffer to extract and/or enrich the trans-membrane proteins or can I go with a normal...

17 November 2013 360 9 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View