I will be performing an experiment on MDA-MB-231 cell line and I would like to know what would be the most efficient dose to arrest cells in G2/M. Thanks!
I am struggling to understand how I can export search results papers from ResearchGate into Excel - could you please advise as soon as possible? All the best, Zuzanna
10 December 2023 1,726 0 View
I'm doing a research on migration process and history of migration and looking for scientific specific data about what kind of challenges migrants can face in foreign countries
04 July 2022 9,508 7 View
Hi, i have a 206 ug/ml stock and I need to prepare a standard curve from it. The first concentration must be 20 ng/ul and each following should be half of the previous, so 10, 5, 2.5 and so on. I...
09 April 2022 5,133 0 View
I am interested in learning more about the main organisations/charities that fund ecological and/or evolutionary projects in various countries (China, United States, India, Germany, Japan, Russia,...
04 August 2021 8,152 6 View
I have a 200 mg/ml ampiciline solution, how much should i add to 300 ml of LB medium to get the final concentration of 40 ug/ml?? How much bacteria (in ml) should i add to 75 ml of medium to...
05 October 2020 6,620 5 View
Hi, anyone tried to culture this cell line? Did you use the flask and media from this producer, or from some other? How about viability? Share any hints please, as I am planning to order this...
12 September 2019 8,517 0 View
Hello, I am looking for literature on methodological aspects of using paper questionnaires in qualitative research. Questionnaire is usually associated with quantitative approach but what with...
30 June 2019 5,590 12 View
I am trying to sequence a PCR product, the length is sufficient, it on agarose gel it gives nice, sharp band, without any smear or clouds of leftover oligos. Concentration measured on...
06 August 2018 5,172 3 View
I got pure phase of magnetite nanoparticles, checed by powdered X- ray diffraction... Even so they were coated, after probe of dispersion by sonication they become brown! The synthesis was well...
20 June 2016 1,566 8 View
We've found a chrysoprase bead in a tumulus grave in Kuwait. The burial is multiple and multi-phase but most of the equipment suggest dating for Neolithic. This bead (biconical, with chamfered...
22 March 2015 5,881 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View