To ascertain the optimal drying duration post-nutrient/ watering application for maximizing spore yield in arbuscular mycorrhizal fungi, should plant be harvested (cut the stem) 1, 2, or 3 weeks following the cessation of nutrient provision, or immediately (1 day) post-nutrient application. And is it necessary to dry outside the host plant? and what is the requisite drying period for achieving the highest spore density?
Yes Harvesting the plant 2 to 3 weeks after stopping nutrient/watering has been shown in several studies to maximize spore production. This interval allows the AM fungi to sense host senescence or stress signals and accelerate sporulation.
Yes, drying outside the host plant is generally necessary. After harvest, gently washing the roots and placing them (or the entire root–soil system) in dry conditions promotes spore maturation and release from hyphae.
Drying within the soil matrix (especially in trap cultures or sand:soil mixtures) is often more effective than drying isolated roots alone.
A drying period of 10 to 14 days at room temperature (around 25–30°C) in a well-ventilated, shaded area is typically sufficient for maximum sporulation.
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