Hi,
I am studying the phase separation of a monoclonal antibodies using a combination of flow imaging techniques as well as brightfield microscopy as a static control. To match the properties of the flowcell used for the flow techniques I opted for uncoated microscopy slides as well to visualize the droplets in the sample. However I got very different results depending on which vendor I chose even though all slides were uncoated. By looking at the surface tension on the different "uncoated" slides I could see a lot of variation as well. For the slides that allowed me to visualize the droplets it seemed like the droplets interacted with the slide and coverslip surface in a repulsive manner as no droplets were vivible in the middle of the slides and all droplets migrated toward the edges of the coverslip and out of it in a few seconds.
So my question is, what are the recommended slide coatings and types (cavity slide, etc) to study LLPS? I have tried the PEG-silane coating which didn't work for me. I have also tried slides with a positive charge and uncoated slides from four different vendors.
Thank you so much for your returns.
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